• Super fast protocol, optimized for same-day transfection
• Effective in a broad range of cell lines
• Ideal for high-throughput applications
• Value priced-your research dollars will go further
• Unique Advanced Conjugation Enhancement (ACE) technology promotes effective delivery with exceptionally low cytotoxicity

GenePORTER^® Gold Transfection Reagent is our newest, highly effective and reproducible lipid based gene delivery formulation for the widest variety of cell lines, especially the easy-to-transfect cell lines. It utilizes our Advanced Carrier Enhancement (ACE) technology that maximizes transfection efficiency and transgene expression levels with minimal cytotoxicity. The GenePORTER Gold Reagent and protocol have been optimized to provide the fastest, easiest, and most cost effective transfection reactions possible. Because of its simplicity and cost advantage, the GenePORTER Gold Transfection Reagent is ideal for high throughput or large scale applications where the need for cost control and great results are highly desired.

GenePORTER Gold Transfection Reagent Kit

• 400 rxns 1.5 ml - T204015
• 800 rxns 2 x 1.5 ml - T204030
• 4000 rxns 15 ml - T204115
• 10 rxns sample kit 0.1 ml - T204001S

Effective molecular target delivery faces many barriers, from crossing the cell type-specific extracellular membrane, to cytoplasmic degradation, to translocation across the nuclear membrane. To be effective, the transfection reagent/DNA complexes must overcome all of these challenges while minimizing cytotoxicity. GenePORTER Gold Transfection Reagent is Genlantis" newest delivery formulation and protocol and the result of extensive R&D in the area of in vitro transfection. GenePORTER Gold Reagent is based on a proven cationic lipid formulation with more than 850 citations to date. The GenePORTER Gold kit incorporates our exclusive ACE technology, which ensures consistent delivery, high quality and reliability, and the lowest cytotoxicity of any other reagent on the market today.

Quick Protocol

GenePORTER Gold Cost Comparison

GenePORTER Gold Comparison Data (click graph to enlarge)

GenePORTER Gold Comparison Images

HEK 293 Cells (click image to enlarge)

HEK 293 Figure Legend:Transfection efficiency in HEK 293 cells. HEK 293 cells were plated on poly-D-lysine coated glass cover slips and transfected with 1ug of gWiz GFP and GenePORTER GOLD (Genlantis), jetPRIME(Polyplus-transfection) or Lipofectamine 2000(Invitrogen, Inc.) in 12 well plates according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then permeablized, and incubated with AlexaFluor� 555 phalloidin (Invitrogen, Inc.), which stains cellular actin red. Cells were mounted on slides with Prolong� Gold antifade reagent with DAPI (Invitrogen, Inc.), which stains the cell nuclei blue.Cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. Transfection efficiency, cell viability and cell morphology are simultaneously assessed indicating both high transfection efficiency and significantly higher viability for GenePORTER GOLD transfected cells relative to cells transfected with jetPRIME and Lipofectamine 2000.

RAW 264.7Cells (click image to enlarge)

RAW 264.7 Figure Legend:Transfection efficiency in hard-to-transfect RAW 264.7 cells. RAW 264.7 cells were plated on glass cover slips and transfected with 1ug of gWiz GFP and GenePORTER GOLD (Genlantis), jetPRIME (Polyplus-transfection) or Lipofectamine 2000(Invitrogen, Inc.) in 12 well plates according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen,Inc.), which stains cellular actin red. Cells were mounted on slides with Prolong Gold antifade reagent with DAPI (Invitrogen,Inc.), which stains the cell nuclei blue. Cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI.Transfection efficiency, cell viability and cell morphology are simultaneously assessed indicating both high efficiency and significantly higher viability for GenePORTER GOLD transfected cells relative to cells transfected with jetPRIME and Lipofectamine 2000.